Components of an Automated ELISA Workstation
The enzyme-linked immunosorbent assay (ELISA) is a test to identify and measure antibodies in the blood. The testing involves many steps, so Dynex Technologies created an automated ELISA processing system for rapid, accurate results. Our workstations have many different components that are essential to testing, including microplates, incubators, plate washers and buffers.
Microplates are the location of the first step of ELISA testing, which is called coating and capture. These wells are where the antigen is secured in place so it can be joined to an antibody linked to a reporter enzyme. This immobilization is essential because it makes it easy to separate the materials according to what binds together (bound) and what does not (unbound).
The DYNEX® Technologies automated process uses 96 microwell plates, which allows many samples to be tested at once. Our plates are designed for ELISA testing to ensure consistency. The uniform plates reduce edge effects while providing the best conditions for the laboratory technician to collect data.
ELISA Microplate Incubators
Incubation occurs after the plate has been coated to allow the coating to absorb into the well’s surface. This absorption is passive and occurs as a result of the interaction between the amino acid side chain in the antigen or antibody and the well’s surface.
DYNEX® Technologies’ automated ELISA system will allow the coating to incubate at the ideal temperature and time, which can vary depending on the testing time frame. Longer times need a lower temperature, while shorter times need a higher temperature. The temperature is also critical when the incubation is stationary.
ELISA Microplate Washers
The ELISA test uses surface binding to separate the materials in the sample. For this reason, plate washing is necessary between each step of testing to wash away unbound materials. The plate washing is repeated three to five times for thorough cleaning, and the excess wash solution is removed during the final washing stage.
Buffers are used during ELISA testing for:
- Coating: A coating buffer incubates the antigen or antibody until it’s absorbed into the well’s surface.
- Blocking: A blocking buffer prevents the binding of detection antibodies to the plate surface.
- Washing: A washing buffer cleans the wells between each step.
- Dilution: The sample must be diluted in a buffer to achieve accurate results due to the nature of blood proteins.
Contact DYNEX® Technologies for More Information Today
ELISA processing systems by DYNEX® Technologies automate the testing steps for savings in labor and costs. During this automated process, the lot numbers and expiration dates are tracked to keep the samples organized and accurate. Automating the ELISA testing process allows laboratory staff to tend to other jobs while waiting for the results, increasing the lab’s efficiency.
Browse the automated ELISA machines on our website. Our design options can accommodate different numbers of samples in microwells, so you can choose the equipment that fits your laboratory’s workflow. For more information about our ELISA workstations, contact us online.